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Purification of the ATase of Actinoplanes sp. strain SN223/29
| Stage | Total protein (mg) | Total activity (nkat) | Sp act (nkat/mg of protein) | Recovery (%) | Purification factor |
|---|---|---|---|---|---|
| Culture filtrate | 400 | 1,740 | 4.3 | 100 | 1.0 |
| Ammonium sulfate precipitation | 139 | 1,147 | 7.7 | 66 | 1.8 |
| DEAE-chromatography | |||||
| Step 1 | 89 | 1,078 | 12.1 | 62 | 2.8 |
| Step 2 | 20 | 789 | 38.8 | 45 | 8.9 |
| Starch adsorption-elution techniques | |||||
| Acarbose elution | 10 | 538 | 55.5 | 31 | 12.8 |
| Maltose elution | 6 | 425 | 77.3 | 24 | 17.8 |
