TABLE 4.
Donor specificity of ATasea
| Homologue or homologue mixture | % Decrease of component | Increase in acarbose concn |
|---|---|---|
| Mixture | ||
| Component 4a | 91 | |
| Component 4b | 91 | Yes |
| Component 4c | 46 | |
| Component 2 | 33 | Yes |
| Mixture | ||
| Component B | 80 | No |
| Pseudo-acarbose | 100 | |
| Component A | 8 | No |
| Component C | 8 | No |
a
Various acarbose homologous or homologue mixtures (16.6 mg/ml) were incubated with maltose (200 mM) and purified ATase (15 nkat/ml) in 0.1 mM Tris-HCl (pH 7.2) plus 0.01 mM CaCl2 for 0 and 24 h. The resulting concentration differences are presented. (See Table 1 for a definition of the homologues other than acarbose).