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. Author manuscript; available in PMC: 2023 Feb 9.
Published in final edited form as: Sci Signal. 2022 Aug 9;15(746):eabn2694. doi: 10.1126/scisignal.abn2694

Fig. 7. Response of KRASQ61-mutant PDAC cell lines to combined inhibition of ERK MAPK cascade and autophagy.

Fig. 7.

(A) PDAC cell lines were stably infected with a lentiviral construct encoding mCherry-EGFP-LC3B and then treated with SCH772984 (ERKi, 1 μM) or DMSO for 24 hours. Fluorescence intensities of mCherry and EGFP were monitored using FACS analysis, and autophagic index is plotted, indicating the ratio of the median fluorescence of mCherry to EGFP. Data are the average of three independent biological replicates. (B) Cell viability of KRASQ61-mutant PDAC cell lines co-treated with SCH772984 (ERKi) and chloroquine (CQ) as assessed by CellTiter Glo. Data are representative of three independent experiments.