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. 2022 Sep 21;13:996026. doi: 10.3389/fimmu.2022.996026

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Copyright © 2022 Xie, Gui, Deng, Chen, Chen, Liu, Ku, Tan, Huang, He, Zhang, Lewis, Chen, Xu, Xu, Huang, Liao, Zhang, An and Zhang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Inhibition of LAIR1 enhances dendritic cell differentiation and dendritic cell- induced T cell proliferation in MLR assay. (A) CD14⁺ monocytes isolated from fresh human PBMCs were cultured with GM-CSF and IL-4 for 2 days. The expression of CD11c on CD14 negative population and CD86 were detected. (B, C) Matured DCs were mixed with allogenic T cells in the MLR assay. Surface CD25 (B) and CFSE based cell proliferation were determined by flow cytometry at 72 hours (C). As Statistical Analysis in Methods stated: * indicates p < 0.05; ** indicates p < 0.01, *** indicates p < 0.001.