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PCR analysis of the deletion in soxF of GBsoxFΔ. Genomic DNA was used as a template for PCR with primers 5 and 6 binding within soxF. Purified PCR assay mixtures (4 μl) were applied to each well. Lanes: 1, HindIII-digested λ DNA; 2, plasmid control pRD123.2; 3, mutant GBsoxFΔ; 4, cointegrate GB17::pRD123.2; 5, GB17.
