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. 2022 Sep 21;13:952262. doi: 10.3389/fimmu.2022.952262

Figure 7.

Figure 7

L-MSCs inhibit NK cell cytolytic function through secretion of soluble HLA-C. The two highly expressed NK cell inhibitors in the L-MSC secretome, soluble HLA-B-Bw4 and HLA-C1 were tested for their role in L-MSC-induced inhibition of NK cell cytolysis. The blockade of the KIR3DL1, receptor for HLA-B-Bw4 did not have an impact (A), while blockade of KIR2DL2/3, receptor for HLA-C1 reversed the NK cell inhibition (B). When NK cells were cultured for 3 days with supernatants of L-MSC cultures (sL-MSC), their cytolytic function was inhibited, whereas BM-MSC or A-MSC culture supernatants (sBM-MSC and sA-MSC, respectively) did not impair the NK cytolytic activity (C). The results are representative of 3 independent experiments for panel A&B, and 2 independent experiments for panel (C) Each experiment was done in triplicates per groups. Paired Student’s t-test was used for the statistical analysis. L-MSC, liver mesenchymal stromal cell; BM-MSC, bone marrow mesenchymal stromal cell; A-MSC, adipose mesenchymal stromal cell; NK, natural killer.