FIG. 1.

Sodalis genome size determination. (A) Bacterial DNA samples embedded in agarose plugs were subjected to CHEF electrophoresis using pulse times of 150 to 200 s for 20 h at 200 V and 14°C to obtain chromosomal DNA devoid of plasmids. Sizes on the left are indicated in kilobases. (B) Sodalis genomic DNA devoid of its plasmid was analyzed by CHEF gel electrophoresis. PmeI and PacI fragments were resolved at pulse times of 18.3 to 26.3 s over 35 h at 200 V. Three different pulse times were used to resolve the SwaI fragments in different size ranges: 18.3 to 26.3 s for 35 h (a), 6.8 to 12.9 s for 33 h (b), and 1 to 6 s and 6 to 15 s for 15 h each (c).