Figure 1.

(A) Serial deletions of the 4.9Kb region of the HO-1 promoter. (B) Luciferase activity of serial deletions of the HO-1 promoter after co-transfection of the reporter construct with a plasmid that expresses vGPCR. The results are expressed as fold induction relative to cells transfected with the 4.9Kb promoter construct. (C) Luciferase activity of a minimum promoter with three ARE sites in tandem (3xARE Luc) after transfection of vGPCR; effect of co-transfection with vGPCR and Nrf2 WT, and effect of co-transfection with vGCPR and Nrf2 DN. The results are expressed as fold induction relative to control cells (transfected with the reporter and an empty vector). (D) Fold-changes of Luciferase mRNA expression were assessed by RT-qPCR in triplicate and are presented as means ± SD. (E) Fold-changes of vGPCR mRNA expression were assessed by RT-qPCR in triplicate and are presented as means ± SD. (F) Luciferase activity of the 3xARE Luc reporter constructs after transfection with plasmids expressing vGPCR, Gα12-QL, Gα13-QL, and RhoA-QL (constitutively active forms of Gα12, Gα13, and RhoA, respectively). The results are expressed as fold induction relative to control cells (transfected with the reporter and an empty vector). (G) Luciferase activity of the 3xARE Luc after co-transfection with vGPCR, Gα12-QL, and Gα13-QL with or without RhoA-N19 (a dominant negative form of RhoA). The results are expressed as fold induction relative to control cells (transfected with the reporter and an empty vector). P-value <0.05 (*). P-value <0.002 (**). P-value <0.0002 (***).