Figure 4.

(A) Western Blot Assays performed in NIH3T3 and NIH3T3_vGPCR were evaluated for Nrf2 levels and Nrf2 phosphorylation using anti-phospho Nrf2 and anti- Nrf2. As loading control we used GAPDH. (B) Western Blot Assays performed in lysates of NIH3T3 and NIH3T3_vGPCR cells were evaluated for activation of ERK using anti-phospho ERK1/2 and anti-ERK2; as a control, we used NIH3T3 cells treated with PDGF 5 minutes. (C) Activation p38 using anti-phospho p38 and anti-p38; as control, we used NIH3T3 cells treated with Anisomycin for 20 minutes. (D) Activation of AKT using anti-phospho AKT and anti-AKT as a control, we used NIH3T3 cells treated with PDGF for 20 minutes. (E) Activation of JNK using anti-phospho JNK and anti-JNK as a control, we used NIH3T3 cells treated with PDGF for 20 minutes.