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Inhibition of ERK1/2 activity block Nrf2 nuclear translocation. (A) Confocal microscopy of NIH3T3 stable cell lines for vGPCR control or treated with PD98059 20uM and incubated with anti-Nrf2 and anti-rabbit FITC. For visualizing the nucleus, propidium iodide was used. Magnification 40X. (B) Quantification of fluorescence intensity for the nucleus/cytoplasm localization of Nrf2 in the different conditions from (A).
