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. 2022 Sep 28;610(7930):205–211. doi: 10.1038/s41586-022-05255-2

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© The Author(s) 2022, corrected publication 2022

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Extended Data Fig. 10 — a, Image-processing workflow for the Spc-treated dataset. The sub-tomogram classification and refinement are similar to those developed for the untreated dataset (Extended Data Fig. 4a), except for exclusion of the manual inspection step after template matching. b, FSC curves for all classes calculated following refinements. c, Local-resolution maps for Spc-treated 70S classes. d, Density corresponding to the Spc molecule (magenta) is clearly resolved in the major 'EF-G, A/P^Spc, P/E' class. Densities from the untreated and PUM-treated data, where Spc is not present, are shown for comparison. e, Models built for the five Spc-treated 70S classes. f, Elongation factors and tRNAs in the five classes. g, The model of 'EF-G, A/P^Spc, P/E' shows Spc binds to the 30S neck region, confirming its role in inhibiting 30S head dynamics and mRNA translocation.