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. 2022 Sep 28;610(7930):205–211. doi: 10.1038/s41586-022-05255-2

Extended Data Fig. 11. Classification, refinement and modelling of ribosomes in PUM-treated cells.

Extended Data Fig. 11

a, Image-processing workflow for the PUM-treated dataset. The sub-tomogram classification and refinement are similar to those developed for the untreated dataset (Extended Data Fig. 4a). b, Distribution of translation states for ribosomes collided with a PUM-stalled RNA polymerase (purple; stalled expressome) and for the remaining ribosomes (green) in the PUM-treated cells. c, FSC curves for all classes calculated after refinements. d-f, Local-resolution maps. g, Models built for PUM-treated 70S classes. h, Elongation factors and tRNAs in the six 70S ribosome classes.