Table 1.
Antibodies labelled with a unique oligonucleotide tag identifier for CITE-seq.
| Application | Marker | Clone | Oligonucleotide tag | Source | Concentration |
|---|---|---|---|---|---|
| Feature barcoding | CD28 | 37.51 | ATTAAGAGCGTGTTG | TotalSeq-C, BioLegend | 1 µg ml–1 |
| Feature barcoding | CD44 | IM7 | TGGCTTCAGGTCCTA | TotalSeq-C, BioLegend | 1 µg ml–1 |
| Feature barcoding | CD62L (L-selectin) | MEL-14 | TGGGCCTAAGTCATC | TotalSeq-C, BioLegend | 1 mg ml–1 |
| Feature barcoding | CD39 | Duha59 | GCGTATTTAACCCGT | TotalSeq-C, BioLegend | 1 µg ml–1 |
| Feature barcoding | CD279 (PD-1) | RMP1-30 | GAAAGTCAAAGCACT | TotalSeq-C, BioLegend | 1 µg ml–1 |
| Feature barcoding | CD366 (TIM-3) | RMT3-23 | ATTGGCACTCAGATG | TotalSeq-C, BioLegend | 1 µg ml–1 |
| Feature barcoding | CD223 (LAG-3) | C9B7W | ATTCCGTCCCTAAGG | TotalSeq-C, BioLegend | 1 µg ml–1 |
| Feature barcoding | CD183 (CXCR3) | CXCR3-173 | GTTCACGCCGTGTTA | TotalSeq-C, BioLegend | 1 µg ml–1 |
| Feature barcoding | CD185 (CXCR5) | L138D7 | ACGTAGTCACCTAGT | TotalSeq-C, BioLegend | 1 µg ml–1 |
| Feature barcoding | CD127 (IL-7Rα) | A7R34 | GTGTGAGGCACTCTT | TotalSeq-C, BioLegend | 1 µg ml–1 |
| Feature barcoding | TIGIT (Vstm3) | 1G9 | GAAAGTCGCCAACAG | TotalSeq-C, BioLegend | 1 µg ml–1 |
| Feature barcoding | CD25 | PC61 | ACCATGAGACACAGT | TotalSeq-C, BioLegend | 1 µg ml–1 |
| Feature barcoding | Ly108 (SLAM-F6) | 330-AJ | CGATTCTTTGCGAGT | TotalSeq-C, BioLegend | 1 µg ml–1 |
| Feature barcoding | CD137 (4-1BB) | 17B5 | TCCCTGTATAGATGA | TotalSeq-C, BioLegend | 1 µg ml–1 |
| Feature barcoding | IL-21R | 4A9 | GATTCCGACAGTAGA | TotalSeq-C, BioLegend | 1 µg ml–1 |