Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Sep 28;25(10):1273–1278. doi: 10.1038/s41593-022-01170-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022, corrected publication 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 1 — (a) Confocal images of TC axons and inputs in V1 labeled with AAV-hSYN-eGFP (green) and immunostained for VGluT2 (magenta). Scale bar, 40 μm. (b) Representative image of tdTomato+ cells (magenta) in the NG2-CreER^T2tdTomato mouse line. Cells of the oligodendrocyte lineage immunostained for Sox10 (cyan) and the OPC marker NG2 (yellow). White circles, OPCs confirmed by co-expression of tdTomato and NG2. Scale bar, 20 μm. Fluorescence scale bar, 40 μm. (c) Quantification of the percentages of tdTomato+ cells that co-stain for Sox10 and NG2. Individual data points and mean ± s.e.m. shown; n = 3 mice per group. (d) High resolution images of tdTomato+ cells (magenta) immunostained for NG2 (green). White arrow, confirmed OPC in which tdTomato and NG2 signal overlap. Compared to other tdTomato+ cells (magenta arrow), OPCs can be distinguished by their bean-shaped somata. Scale bar, 10 μm. (e) Confocal images of tdTomato+ cells (magenta) co-localizing with blood vessels (CD31, green). These are likely pericytes and are easy to distinguish from OPCs based upon morphology. Scale bar, 10 μm. (f) High magnification image of a tdTomato+ OPC (magenta) immunostained for NG2 (green). Bottom, volumetric reconstructions of the same cell based upon tdTomato versus NG2 signal, demonstrating a high level of overlap between the two channels. Scale bar, 10 μm.