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. 2022 Sep 19;54(10):1527–1533. doi: 10.1038/s41588-022-01177-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 6 — a, Schematic depicting the trends in ecDNA copy number frequency and copy number under neutral and positive selection. b, Simulations showing ecDNA prevalence in populations derived from a single ecDNA+ cell with ecDNA under positive or neutral selection. Positive selection s = 3; neutral s = 1. c, Quantification of ecDNA frequency in patient and cell line samples grouped by amplified oncogene compared to expected levels given varying levels of selection as indicated. d, Quantification of ecDNA numbers at Day 6 and Day 10 after CRISPR cutting of regions of the COLO320-DM genome, either on or off of ecDNA. Shows clear evidence for selection of ecDNA both by the severe drop in copy number when targeted and the inidcation that the copy number begins to return to initial levels. Note ecDNA_MYC at day 6 is severely limited in its growth and only 6 metaphases were able to be identified and imaged. P-values shown from 2 sided t-tests.