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. 2022 Sep 19;54(10):1527–1533. doi: 10.1038/s41588-022-01177-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 8 — a, Treatment of long term palbociclib resistant populations of TR14 cells with palbociclib or abemaciclib, showing resistance to treatment. b, Treatment of long term abemaciclib resistant populations of TR14 cells with palbociclib or abemaciclib showing resistance to treatment. c, Validation of increased ecDNA copy number by qPCR for CDK4. (a-c) Data presented as mean +/− standard deviation from 3 technical replicates. d, Crystal violet staining of TR14 cells re-challenged with palbociclib or abemaciclib after development of resistance, or not (DMSO). e, Quantification of d showing resistance in populations treated with CDK4 inhibitors for 60 days. Data presented as mean +/− standard deviation from 3 technical replicates.