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. 2022 Sep 19;54(10):1527–1533. doi: 10.1038/s41588-022-01177-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 3 — a, Representative images of PC3 parental and PC3-TetO cell lines showing extensive MYC amplification on both. PC3-TetO shows significant TetO FISH signal on multiple ecDNA bodies as well. b, PCR amplification of 96-mer TetO repeats. c, PCR amplification of 96-mer TetO repeats from DNA isolated from PC3-TetO cells confirming insertion. d, Sanger sequencing of PCR amplification product from PC3-TetO cells. Both left and right junctions were repaired by homologous recombination at the insertion site.