Extended Data Fig. 8. Developmental trajectories, timing of mesoderm differentiation and expression of selected genes.
a. Latent time for epiblast and mesodermal derivatives for time series in inDrops sequencing dataset. b. Quantification of the latent time analysis for epiblast and all the mesodermal derivatives for all time points analysed in the inDrops sequencing dataset. c. Annotated UMAP of the tiny sci-RNA-seq dataset highlighting the paraxial mesoderm cluster. d. Expression of somite markers Meox1, Meox2 and Pax3 in natural embryos and ETiX embryoids (tiny sci-RNA-seq dataset). e. Expression of cardiac markers Hand1, Hand2, atrial differentiation marker Nr2f2, ventricular differentiation marker Irx4, first heart field markers Tbx5, Hcn4, Nkx2.5 and second heart field marker Isl1 in natural embryos and ETiX embryoids (tiny sci-RNA-seq dataset). f. UMAP showing individual timepoints for natural embryos and ETiX embryoids in combined UMAP presented in Fig. 4m