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. 2022 Aug 25;610(7930):143–153. doi: 10.1038/s41586-022-05246-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 11 — a–c ETiX embryoid (a) at day 7 of development after stationary culture and natural embryo (b) at E8.0 of development stained to reveal STELLA, NANOG and SOX2 to highlight presence of committed PGCs (n = 2 ETiX7 from 2 independent experiments, n = 4 embryos). Boxes are magnified below (a,b) and on the right (c). Scale bars, 100 μm for main panel, 50 μm for magnified boxes. d. Day 8 ETiX-embryoid stained to reveal STELLA, NANOG and SOX2 highlighting presence of committed PGCs. Scale bar for a-d, 100 μm. (n = 4 ETiX8 from 3 experiments).