Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Aug 25;610(7930):143–153. doi: 10.1038/s41586-022-05246-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — a,b, Ventral view (main image) of day 7 ETiX embryoids after static culture (a) and E8.0 natural embryo (b) showing SOX1-positive neural folds and neural tube extending from the anterior towards the Brachyury-positive posterior. Optical yz (bottom) and xz (right) sections show notochord lying below the neural tube (n = 11 day 7 ETiX embryoids from 4 experiments, n = 3 E8.0 embryos). Scale bars, 100 μm c, Dot plot showing average levels and proportion of cells expressing indicated genes in selected tissues of natural embryos and ETiX embryoids (from inDrops scRNA-seq data). Epi, epiblast. d,e, Lateral view of day 8 ETiX embryoid (d) and E8.5 natural embryo (e) showing FOXG1 in telencephalon and OTX2 in forebrain and midbrain (n = 4 ETiX8 from 3 experiments; n = 2 E8.5 embryos). Scale bars, 100 μm. f, Coronal view of neural tube sections showing dorso–ventral patterning in day 8 ETiX embryoids. Sections reveal pan-neural markers (SOX1 and SOX2), dorsal markers (PAX6 and PAX3), ventral markers (FOXA2, OLIG2 and NKX2-2) and neural crest markers (SOX10 and PAX3). Scale bars, 50 μm. n = 3 ETiX8 from 3 experiments. g, Subclustered UMAP of neural progenitors highlighting neural subtypes from tiny-sci-RNA-seq. h, Individual UMAPs showing the contribution of each timepoint to global UMAP in g. i, The proportion of cell types in g in each individual day 8 ETiX embryoid sequenced by tiny-sci-RNA-seq. MHB, midbrain–hindbrain boundary.

Source data