Fig. 3. Pax6 knockout in ETiX embryoids recapitulates known mouse embryonic phenotypes.
a, Coronal sections of wild-type (WT) and Pax6-knockout (KO) ETiX embryoids stained to reveal dorsal and ventral neural tube markers. Scale bar, 50 μm. b, Quantification of images represented in a, showing no significant difference in SOX1-positive cell number in the neural tube but an increased proportion of NKX2-2-positive cells following Pax6-knockout (3 control day 8 ETiX and 4 day 8 Pax6-KO ETiX from 3 experiments). Violin plots show median and quartiles. Two-sided Mann–Whitney U-test, *P < 0.05. For SOX1-positive cells, P = 0.5382; for NKX2-2 positive cells, P = 0.0135. c, Gene Ontology (GO) analysis of genes enriched in 2 Pax6-knockout ETiX embryoids at day 8 (tiny-sci-RNA-seq) compared with 5 ETiX day 8 controls. NS, not significant (P > 0.05).