Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Aug 25;610(7930):143–153. doi: 10.1038/s41586-022-05246-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — a,b, Lateral view of day 8 ETiX embryoid (a) and natural E8.5 embryo (b) showing SOX2, Brachyury (BRY) and DNA (DAPI), highlighting NMPs in the tail bud region (n = 5 ETiX8 from 4 experiments, n = 3 embryos). Scale bars, 100 μm. Inset, schematic view. c,d, Dorsal view of day 7 ETiX embryoid after stationary culture (c) and natural E8.0 embryo (d) showing SOX2, HOXB4 and DNA, highlighting somite formation flanking neural tube. Right, magnified view of outlined region containing somites (n = 9 day 7 ETiX embryoids from 4 experiments, n = 5 E8.0 embryos). Inset, schematic view. Scale bars a-d, 100 μm (main image), 50 μm (magnified view a–c), 20 μm (magnified view d). e, Quantification of somite pairs in natural E8.0 embryos and day 7 ETiX embryoids. Violin plots show median and quartiles. Two-sided Mann–Whitney U-test, P = 0.3020. f, Somite area of E8.0 embryos and day 7 ETiX embryoids. Violin plots show median and quartiles. Two-sided Mann–Whitney U-test. P = 0.2717. For e,f, n = 9 day 7 ETiX embryoids from 4 experiments, n = 5 E8.0 embryos. g,h, Day 8 ETiX embryoid (lateral view) (g) and natural E8.75 embryo (h) (lateral view) showing OTX2, MYH2 and GATA4, highlighting heart (n = 8 ETiX8 from 3 experiments, n = 2 natural embryos). Outlined areas are magnified on the right. Scale bars, 100 μm (main image), 20 μm (magnified view). i, Schematic of mouse heart at E8.5, indicating location of sections. j, Coronal sections of ETiX embryoid at day 8 showing GATA4, NKX2-5 and MYH2. Scale bar, 100 μm. n = 3 ETiX8 from 3 independent experiments. k, Dot plot showing levels and proportion of cells expressing indicated genes in indicated tissues from natural embryos (NE) and ETiX embryoids by inDrops scRNA-seq. l, Velocity plots for epiblast and mesodermal derivatives for time series in the inDrops sequencing dataset. m, UMAP of the tiny-sci-RNA-seq dataset, showing cell types in the subclustered cardiac lineage.

Source data