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Purification of ethylbenzene dehydrogenase from Azoarcus sp. strain EB1
| Purification step | Protein amt (mg) | Total activity (nmol min−1) | Sp act (nmol min−1 mg of protein−1) | Yield (%) | Purification (fold) |
|---|---|---|---|---|---|
| Cell extract | 16.5 | 109 | 6.6 | 100 | 1 |
| Solubilization and acetone precipitation | 7.4 | 36 | 4.8 | 33 | 0.7 |
| Cation-exchange chromatography | .08 | 3.8 | 47 | 3.5 | 6.8 |
