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. 2022 Oct 5;13:5871. doi: 10.1038/s41467-022-33323-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a GV oocytes from Bnc1^+/+ (n = 9) and Bnc1^tr/tr (n = 9) mice at 4 weeks old (p value = 0.0032). b GV oocytes from Bnc1^+/+ (n = 3) and Bnc1^tr/tr (n = 3) mice at 12 weeks old (p value=0.4909). c Comparison of the numbers of primordial follicles (PrFs) of mice (n = 3) at PD1 (p value = 0.6174). d Comparison of the numbers of PrFs, primary follicles (PFs), secondary follicles (SFs) and antral follicles (AFs) of mice (n = 3) at 3 weeks old (p value = 0.0093 for PrFs, p value = 0.0903 for PFs, p value = 0.9641 for SFs, p value = 0.0804 for AFs). e Comparison of the follicle numbers of mice (n = 3) at 4 weeks old (p value = 0.0183 for PrFs, p value = 0.8694 for PFs, p value = 0.0769 for SFs, p value = 0.1996 for AFs, p value = 0.3926 for AtFs). f Comparison of the follicle numbers of mice (n = 4 for Bnc1^+/+, n = 3 for Bnc1^tr/tr) at 12 weeks old (p value = 0.0211 for PrFs, p value = 0.0910 for PFs, p value = 0.0560 for SFs, p value = 0.0571 for Afs, p value = 0.0021 for AtFs). g Comparison of the follicle numbers of mice (n = 3) at 16 weeks old (p value = 0.01810 for PrFs, p value = 0.2460 for PFs, p value = 0.1346 for SFs, p value = 0.2720 for AFs, p value = 0.0077 for AtFs). Scale bar = 500 μm. h Comparison of the growing follicle numbers of mice (n = 3) at 3, 4, 12, and 16 weeks old (p value = 0.3265 for 3 weeks old, p value = 0.6693 for 4 weeks old, p value = 0.0750 for 12 weeks old, p value = 0.1923 for 16 weeks old). i Comparison of the ratios of growing follicle numbers to PrFs of mice (n ≥ 3) at 3, 4, 12, and 16 weeks old (p value = 0.1352 for 3 weeks old, p value = 0.0341 for 4 weeks old, p value = 0.0592 for 12 weeks old, p value = 0.0040 for 16 weeks old). The error bars indicate the mean values ± SDs, unpaired t test, two-tailed, *p value < 0.05, **p value < 0.01 and ***p value < 0.001. Source data are provided as a Source Data file.