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. 2022 Oct 5;13:5871. doi: 10.1038/s41467-022-33323-8

Fig. 3. Bnc1 mutation induces follicular atresia through nonapoptotic cell death.

Fig. 3

a Ovaries were obtained from Bnc1+/+ (n = 3) and Bnc1tr/tr mice (n = 3) at 16 weeks old for western blotting (WB). The expression levels of P53, Bcl2, Bax, PARP, Caspase3 and cleaved-caspase3 are shown (p value = 0.6585 for P53, p value = 0.0457 for Bcl2, p value = 0.3090 for Bax, p value = 0.3467 for BAX/BCL2, p value = 0.0624 for Caspase3, p value = 0.4892 for cleaved-Caspase3/Caspase3 and p value = 0.4162 for PARP). b GV oocytes were obtained from Bnc1+/+ (n = 3) and Bnc1tr/tr (n = 3) mice at 4 weeks old for real-time PCR. The mRNA expression of Bax and Bcl2 is shown (p value = 0.7446 for Bax, p value = 0.0241 for Bcl2 and p value = 0.0247 for Bax/Bcl2). c GV oocytes obtained from Bnc1+/+ and Bnc1tr/tr mice at 4 weeks old were used for detection of early apoptosis (3 independent experiments with total oocyte numbers >30 oocytes). d GV oocytes obtained from Bnc1+/+ and Bnc1tr/tr mice at 4 weeks old were used for detection of Caspase3 (CAS3) and the DNA damage marker γ-H2AX (3 independent experiments with total oocyte numbers >30 oocytes). The error bars indicate the mean values ± SDs, unpaired t test, two-tailed, *p value < 0.05, **p value < 0.01 and ***p value < 0.001. Source data are provided as a Source Data file.