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. 2022 Oct 5;13:5871. doi: 10.1038/s41467-022-33323-8

Fig. 6. Bnc1 truncation mutation sensitizes oocytes to ferroptosis by regulating NF2-YAP signaling.

Fig. 6

a ChIP–qPCR for BNC1 at Nf2 promoter using mouse ovaries (n = 5, p value < 0.0001, 3 independent experiments). b Dual luciferase reporter assay of Bnc1 at the Nf2 promoter (n = 6, the error bars indicate the mean values ± SDs, unpaired t test, two-tailed, p value < 0.0001, 3 independent experiments). c NF2 and active YAP in GV oocytes at 4 weeks old (the error bars indicate the mean values ± SDs, unpaired t test, two-tailed, p value < 0.0001 for NF2, p value < 0.0001 for nuclear/cytoplasmic YAP, 3 independent experiments with total oocyte numbers >30 oocytes). d RT–PCR of Nf2, Tfrc and Acsl4 in GV oocytes (n = 3) at 4 weeks old (p value = 0.3555 for Nf2, p value < 0.0001 for Tfrc and p value < 0.0001 for Acsl4). e TFRC of GV oocytes at 4 weeks old (the error bars indicate the mean values ± SDs, unpaired t test, two-tailed, p value < 0.0001, 3 independent experiments with total oocyte numbers >30 oocytes). f IHC of NF2, active YAP and TFRC in mouse ovaries at 8 weeks old (n = 3). g WB of NF2, p-YAP, active YAP, YAP, ACSL4 and TFRC in GV oocytes at 4 weeks old (3 independent experiments). h Fe2+ in GV oocytes at 4 weeks old (p value = 0.0010, the error bars indicate the mean values ± SDs, unpaired t test, two-tailed, p value < 0.0001, 3 independent experiments with total oocyte numbers >30 oocytes). i Heatmap of targeted lipidomics of Bnc1+/+ and Bnc1tr/tr GV oocytes(n = 6). Source data are provided as a Source Data file.