Table 2.

Percent viability of cell suspensions of C. roseus before and after cryopreservation in sorbitol containing media.

Treatments	Viability %
	0.2 M	0.4 M		0.6 M
	− LN	− LN	+ LN	− LN	+ LN
Control	97.85 ± 0.29a	93.18 ± 0.75a	0.0 ± 0.0e	84.86 ± 0.23a	0.0 ± 0.0f
5% PEG	83.18 ± 3.20b	78.80 ± 2.18b	46.12 ± 1.81a	75.12 ± 2.71b	38.2 ± 1.32a
10% PEG	70.55 ± 1.88c	69.12 ± 1.16c	33.25 ± 2.66b	61.22 ± 2.17c	31.36 ± 2.30b
5% PEG + 5% DMSO	58.74 ± 2.18e	54.64 ± 2.14d	28.94 ± 1.54c	51.04 ± 1.14d	26.62 ± 2.21c
5% PEG + 10% DMSO	42.45 ± 3.12f	38.22 ± 2.70f	21.14 ± 2.17d	31.42 ± 2.18f	24.66 ± 1.42c
5% PEG + 5% glycerol	63.44 ± 2.18d	56.77 ± 2.12d	27.26 ± 1.40c	45.11 ± 2.61e	19.74 ± 1.50d
5% PEG + 10% glycerol	45.56 ± 3.17f	41.15 ± 1.46e	21.33 ± 1.21d	32.46 ± 2.14f	12.88 ± 2.16e

Cell suspension was cultured in liquid medium with 0.2–0.6 M sucrose for 2 days (osmoprotectant treatment) followed by 2 h cryoprotecant treatment and two-step cryopreservation.

Absence of column (0.2 M/ + LN) corresponds to zero viability of cells in all cryopreserved treatments. Values are means ± standard errors of 3 replicates of two experiments.Mean values within a column followed by different letters are significantly different at p = 0.05 according to Duncan’s Multiple Range Test (DMRT).

− LN before cryopreservation, + LN after cryopreservation.