Table 3.
Regrowth of C. roseus embryogenic cultures cryopreserved following different osmoprotection and cryoprotection treatments.
| Osmoprotectant | Cryoprotectant | Number of Petri dishes with regrowth/regrowth % | Mean number of cell colonies grown per Petri dish |
|---|---|---|---|
| 0.4 M sucrose | CP-A | 7/77.7a | 9.26 ± 1.01a |
| CP-B | 2/22.2c | 3.11 ± 0.18b | |
| CP-C | 3/33.3b | 3.67 ± 1.12b | |
| CP-D | 1/11.1d | 1.29 ± 0.42d | |
| CP-E | 3/33.3b | 2.72 ± 0.08c | |
| CP-F | 2/22.2c | 2.15 ± 0.18c | |
| 0.6 M sucrose | CP-A | 5/55.5a | 7.18 ± 1.21a |
| CP-B | 2/22.2c | 2.16 ± 0.44c | |
| CP-C | 3/33.3b | 3.10 ± 1.11b | |
| CP-D | 2/22.2c | 2.12 ± 0.26c | |
| CP-E | 3/33.3b | 3.68 ± 0.61b | |
| CP-F | 1/11.1d | 0.95 ± 0.01d | |
| 0.4 M sorbitol | CP-A | 5/55.5a | 5.83 ± 1.32a |
| CP-B | 3/33.3c | 3.14 ± 1.10c | |
| CP-C | 2/22.2d | 2.10 ± 0.22c | |
| CP-D | 2/22.2d | 2.06 ± 0.16c | |
| CP-E | 4/44.4b | 4.11 ± 1.14b | |
| CP-F | 2/22.2d | 1.22 ± 0.18d | |
| 0.6 M sorbitol | CP-A | 3/33.3a | 1.65 ± 0.13a |
| CP-B | 1/11.1c | 0.26 ± 0.05e | |
| CP-C | 2/22.2b | 1.12 ± 0.08b | |
| CP-D | 1/11.1c | 0.53 ± 0.11d | |
| CP-E | 2/22.2b | 1.12 ± 0.20b | |
| CP-F | 1/11.1c | 0.66 ± 0.05c |
Data were recorded 4 weeks after rewarming.
Nine Petri dishes were used and the number of Petri dishes showing cell regrowth was recorded.
Values are means ± standard errors of 3 replicates of two experiments. Mean values within a column followed by different letters are significantly different at p = 0.05 according to Duncan’s Multiple Range Test (DMRT).