Table 6.

Number of embryos at different developing stages in optimized cryopreservation procedure (cryoprotectant treatments with 2 h dehydration time) in C. roseus cell culture.

Cryoprotectant treatment	Embryo development stages
	Globular	Heart	Torpedo	Cotyledonary
Control	61.5 ± 1.18a	22.5 ± 1.2a	9.00 ± 0.80c	6.25 ± 1.7a
5% PEG (CP-A)	54.33 ± 2.27b	21.11 ± 2.34b	14.34 ± 1.34a	6.61 ± 0.89a
10% PEG (CP-B)	52.78 ± 1.85c	19.23 ± 1.67c	12.55 ± 1.67b	5.38 ± 1.54b

Optimized MS medium contained NAA (0.5 mg/l) + BAP (1.5 mg/l). Values are means ± standard errors of 3 replicates of two experiments. Mean values within a column followed by different letters are significantly different at p = 0.05 according to Duncan’s Multiple Range Test (DMRT).