TABLE 1.
Bacteria and plasmids used
| Strain or plasmid | Genotype or descriptiona | Reference or sourceb |
|---|---|---|
| Strains | ||
| AB259 | supQ80λ− relA1 spoT1 thi-1 | CGSC |
| SBG231 | AB259 sjmA1; spontaneous MccJ25r mutant | This study |
| SBG231recA | SBG231 Δ(recA-srl)306 srl-301::Tn10-84 | This study |
| χ342 | proC29 relA1 spoT1 metB1 bglF18::IS150 | CGSC |
| AB1133 | F−thr-1 ara-14 leuB6 lacY1 Δ(gpt-proA)62 supE44 galK2 λ− rac hisG4 rfbD1 rpsL31 kdgK51 xyl-5 mtl-1 argE3 thi-1 | CGSC |
| SBG232 | SBG231 rpoB | This study |
| CSH126 | Δ(recA-srl)306 srl-301::Tn10-84 | CSHL |
| Plasmids | ||
| pDJJ11 | pBR322 carrying rplJL rpoB | C. Gross |
| pDJJ12 | pBR322 carrying rplJL rpoB rpoC htrC | C. Gross |
| pMADC2 | pDJJ12 carrying the rpoC931 mutation (MccJ25r) | This study |
| pGC01 | pBR322 carrying fhuA | J. Coulton |
| pTUC347 | pUC18 carrying mcjABC; MccJ25+ ImmJ25− | 30 |
| pJS200 | pACYC184 carrying mcjD; MccJ25− ImmJ25+ | 29 |
| pGEM-4Z | Apr; cloning vector | Promega |
a
MccJ25r, resistant to microcin J25; MccJ25+, MccJ25 producer; ImmJ25+, immune to MccJ25.
b
CGSC, E. coli Genetic Stock Center; CSHL, Cold Spring Harbor Laboratory.