Figure 5.

PNP hydrogels mitigate gross toxicity caused by CD40a therapy. CD40a dose was titrated and treatment‐induced weight loss was observed. Area under the curve analysis of body mass curves during the acute weight loss phase (5 days post treatment) for mice bearing A) MC38 and B) B16F10 tumors. Statistical comparisons performed with a one‐way ANOVA, multiple testing error controlled using the FDR approach (Q = 5%). Histopathology of the liver and spleen were assessed in B16F10 mice, 3 days after treatment. C) Quantification of degree of liver necrosis. D) Representative images of livers and spleens from treated mice. Scale bars indicate 100 microns. Results are from three independent experiments. N = 8–10 for (A), n = 4–5 for (B), and n = 5 for (C–E).