Reagent or Resource	Source	Identifier
Goat anti‐Rabbit IgG (H+L) Highly Cross‐Adsorbed Secondary Antibody, Alexa Fluor 555	Invitrogen	A21429
Bacterial strand
E. coli NEB 5‐alpha	New England Biolabs (NEB)	C2987H
Chemicals
Cholesterol	Sigma‐Aldrich	C8667
Pregnenolone	Aladdin	P129412
4,7,10‐trioxa‐1,13‐tridecanediamine	Sigma‐Aldrich	369519
NaBH(OAc)3	Sigma‐Aldrich	316393
1,2‐dichloroethane	Sigma‐Aldrich	284505
Triethylamine	Sigma‐Aldrich	471283
Ethanolamine	Sigma‐Aldrich	E9508
Pravastatin sodium	Sigma‐Aldrich	P4498
Methyl‐β‐cyclodextrin	Sigma‐Aldrich	332615
Sulfo‐NHS‐SS‐Biotin	APExBIO	A8005
SNAP‐Surface 549	New England Biolabs (NEB)	#S9112S
SNAP‐Cell Oregon Green	New England Biolabs (NEB)	#S9104S
Cycloheximide	Sigma‐Aldrich	5087390001
2‐Bromohexadecanoic acid	Sigma‐Aldrich	21604
Triethanolamine	Sigma‐Aldrich	V900257
Methoxypolyethylene glycol maleimide	Sigma‐Aldrich	712469
Hydroxylamine	Sigma‐Aldrich	438227
N‐Ethylmaleimide	Sigma‐Aldrich	E3876
Tris(2‐carboxyethyl) phosphine hydrochloride	Sigma‐Aldrich	C4706
IWP‐2	Sigma‐Aldrich	I0536
CHIR99021	Cayman	13122
Tunicamycin	Aladdin	T101151
7β‐Hydroxycholesterol	Aladdin	C130187
25‐Hydroxycholesterol	Aladdin	C130176
22(R)‐Hydroxycholesterol	Sigma‐Aldrich	H9384
5‐Cholesten‐3β‐ol‐7‐one	Sigma‐Aldrich	C2394
22(S)‐Hydroxycholesterol	Sigma‐Aldrich	H5884
Cholesterol 5β,6β‐epoxide	Sigma‐Aldrich	C2648
Thiazolyl Blue Tetrazolium Bromide	Sigma‐Aldrich	M5655
Hoechst	Thermo	1990363
Puromycin	Invivogen	ant‐pr‐1
Geneticin	Invivogen	ant‐gn‐1