Reagent or Resource	Source	Identifier
GAPDH‐RT‐qPCR primers	This study	F: GGAGCGAGATCCCTCCAAAAT R: GGCTGTTGTCATACTTCTCATGG
Recombinant DNA
Lenti‐EF1α‐puro V5‐Fzd1	This study
Lenti‐EF1α‐puro V5‐Fzd2	This study
Lenti‐EF1α‐puro V5‐Fzd3	This study
Lenti‐EF1α‐puro V5‐Fzd4	This study
Lenti‐EF1α‐puro V5‐Fzd5	This study
Lenti‐EF1α‐puro V5‐Fzd6	This study
Lenti‐EF1α‐puro V5‐Fzd7	This study
Lenti‐EF1α‐puro V5‐Fzd8	This study
Lenti‐EF1α‐puro V5‐Fzd9	This study
Lenti‐EF1α‐puro V5‐Fzd10	This study
Lenti‐EF1α‐puro V5‐Smo	This study
Lenti‐EF1α‐puro V5‐SNAP‐Fzd5	This study
Lenti‐EF1α‐puro V5‐SNAP‐Fzd7	This study
Lenti‐EF1α‐puro HA‐Tmem79	This study
Lenti‐EF1α‐puro HA‐Shisa3	This study
Lenti‐EF1α‐puro Flag‐Sec23B	This study
Lenti‐EF1α‐puro His6‐V5‐Fzd5	This study
Lenti‐EF1α‐puro His6‐V5‐Fzd7	This study
Lenti‐EF1α‐puro Flag‐LDLR	This study
pCMV‐VSV‐G	Addgene	#8454
psPAX2	Addgene	#12260
pLKO‐puro shLDLR	This study
Lenti‐Cas9‐puro sgFZD5	This study
pLKO‐puro shZNRF3	This study
pCS2	This study
Software and algorithms
Graphpad Prism 8	Graphpad software	https://www.graphpad.com
ImageJ	[ 26 ]	https://imagej.nih.gov/ij/
PyMol Educational	PyMOL by Schrödinger	https://pymol.org/edu/?q=educational/
PhotoShop CC2019	Adobe	https://www.adobe.com/cn/products/photoshop.html
Origin 2019	OriginLab Corporation	https://www.originlab.com
Other
α‐V5 Agarose Affinity Gel	Sigma‐Aldrich	A7345
Affigel‐10 beads	Bio‐Rad	#1085
Pierce Streptavidin Agarose	Pierce	20349
α‐HA Agarose Affinity Gel	Sigma‐Aldrich	A2095
α‐Flag Agarose Affinity Gel	Sigma‐Aldrich	4596
Ni‐NTA resin	Qianchunbio	A41002‐05