Figure 5.

ABL promotes GC cell growth and multidrug resistance in vivo. A) GC organoids were infected with ABL overexpression vectors or a control lentivirus and treated with or without DDP (5 µg mL⁻¹) or PTX (0.4 µg mL⁻¹) for 24 h. Representative bright‐field images and hematoxylin and eosin (H&E) staining are shown (scale bars = 500 µm, n = 3). B) Sections of organoids were stained with ABL probes and an anti‐cleaved caspase‐3 antibody (scale bars = 50 µm). C) Overexpression of ABL effectively promoted subcutaneous GC tumor growth and antagonized the antitumor effect of cisplatin in nude mice (n = 6). D) Tumor volume was monitored every other day, and tumor growth curves were generated. E) Tumors were extracted and weighed at the end of the experiment. F) The expression of ABL was detected in each group by qRT‐PCR. G) Sections of tumors were stained with H&E, and anti‐Ki67 antibody, an anti‐cleaved caspase‐3 antibody, or TUNEL reagents (scale bars = 50 µm). The data were analyzed by one‐way ANOVA test followed by Turkey's multiple comparisons (D). The data were analyzed by a two‐tailed unpaired Student's t‐test (E and F). The data are represented as the means ± SEM of three independent experiments. * p < 0.05; ** p < 0.01; *** p < 0.001, NS, no significance.