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. 2022 Sep 2;54(9):1472–1485. doi: 10.1038/s12276-022-00844-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a–d Western blot analysis showing the protein expression levels of OGT and O-GlcNAc in human NP cells isolated from NP tissues. The relative band densities were quantified. The two groups were matched by sex. M: male, F: female; n = 12. e–h Representative images showing IHC staining for OGT and O-GlcNAc in human NP tissues. Typical staining in the boxed areas of the images is magnified and shown in the lower rows. The relative mean optical densities were quantified; n = 12. (i, j, l, m) NP cells were subjected to ND for different durations (0, 6, 12, 24, and 36 h). Western blots showing the levels of OGT protein expression and O-GlcNAc. The relative band densities were quantified. k, n After treatment with ND for 36 h, NP cells were labeled with anti-OGT and anti-O-GlcNAc antibodies, and representative fluorescence images are shown. Nuclei were stained with DAPI; scale bar: 50 μm. The data are presented as the mean ± SD values. ^∗∗P < 0.01, ^∗P < 0.05.