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. 2022 Sep 2;54(9):1472–1485. doi: 10.1038/s12276-022-00844-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, b After transfection with FAM134B-siRNA for 48 h, OGT-overexpressing NP cells were subjected to ND for 36 h. Western blot analysis showing the protein expression levels of FAM134B, LC3, and p62, and the relative band densities were quantified. c NP cells were handled as indicated in a. Representative results of ER-Tracker Green and LysoTracker Red staining showing the colocalization profiles of the ER and lysosomes; scale bar: 20 μm. d, e NP cells were handled as indicated in a. Western blot analysis showing the protein expression levels of p53, p21, p16, Bcl-2, Bax, and cleaved caspase 3, and the relative band densities were quantified. f, g NP cells were treated as indicated in a. Representative immunofluorescence results showing p16 and cleaved caspase 3 expression in NP cells. Nuclei were stained with DAPI; scale bar: 100 μm. The data are presented as the mean ± SD values. ^∗∗P < 0.01, ^∗P < 0.05.