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. 2022 Sep 21;54(9):1563–1576. doi: 10.1038/s12276-022-00853-6

Fig. 6. PLD1 inhibition attenuates tumor formation, downregulates ‘do not eat-me’ signals and upregulates ‘eat-me’ signals in colitis-associated CRC.

Fig. 6

a Schematic view of the colitis-associated cancer model (top). AOM/DSS-treated mice were treated with either vehicle or A3373 (10 mg/kg) three times a week for 6 weeks, and AOM/DSS-vehicle (n = 12) or AOM/DSS-A3373 (n = 12) mice were sacrificed at 110 days. H&E staining of the entire Swiss roll of the colon intestine (bottom). b, c The number of visible polyps in the colon intestine. d The indicated mice were followed for long-term survival. The survival probability was analyzed using Kaplan–Meier analysis. e IHC for active caspase-3, Ki67 and CD31 in tumor tissues from vehicle- or A3373-treated mice. Scale bar, 20 µm. f IHC of the indicated phagocytosis checkpoints in the tumor tissues. Scale bar, 20 µm. Representative images were selected from at least six different fields. ***P < 0.001.