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DNase I footprinting assays of oxidized OxyR binding to the top and bottom strands of the yfdI gene. The regions protected by OxyR on both strands are indicated by the brackets. The 180-bp BamHI-EcoRI fragment of pGSO130 was labeled with 32P at either the BamHI site (top strand) or the EcoRI site (bottom strand). The samples were run in parallel with Maxam-Gilbert G/A sequencing ladders.
