FIG. 5.

Heat-induced mutations are dependent on oxygen and are repaired by the oxidative glycosylases Ntg1p, Ntg2p, and Ogg1p. Cultures of Y433 (wild type) (A) and (ntg1ntg2ogg1) (B) JDY47 were grown for 66 h in YPAD before being split. One half of each culture was assayed following heat stress for forward mutation at the CAN1 locus in the presence of oxygen (open symbols); the other half was assayed in the absence of oxygen after introduction to the anaerobic chamber (closed symbols). Results are the means of three experiments ± SD.