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. 2022 Sep 10;101(12):102169. doi: 10.1016/j.psj.2022.102169

Figure 2.

Figure 2

Identification of cTGFBR2 transcript isoforms. (A) Schematic structure of 6 possible cTGFBR2 transcript isoforms and schematic location of the 6 transcript isoform-specific primer sets used for the identification of cTGFBR2 transcript isoforms. (B) RT-PCR identification of cTGFBR2 transcript isoforms. RT-PCR was performed on pooled cDNA to identify cTGFBR2 transcript isoforms with the 6 transcript-specific primer sets. RT-PCR products were analyzed by 2% agarose gel electrophoresis. M, marker. Lane 1, RT-PCR with primer set TGFBR2-1-F and TGFBR2-1-R; Lane 2, RT-PCR with primer set TGFBR2-1-F and TGFBR2-2-R; Lane 3, RT-PCR with primer set TGFBR2-1-F and TGFBR2-3-R; Lane 4, RT-PCR with primer set TGFBR2-2-F and TGFBR2-1-R; Lane 5, RT-PCR with primer set TGFBR2-2-F and TGFBR2-2-R; Lane 6, RT-PCR with primer set TGFBR2-2-F and TGFBR2-3-R.