Table 1.
The primers used in the present study.
| Type | Primer name | Primer sequence |
|---|---|---|
| RACE primer | 5′RACE-GSP1 | R: ACAGCGAGATGTCATTTCCCAGA |
| 5′RACE-GSP2 | F: TTTCCCAGAGGACCAAAGC | |
| 3′RACE-GSP1 | F: CTCCATGGCTTTGGTCCTCTG | |
| 3′RACE-GSP2 | F: CTCGCTGTAATGGTGTTGG | |
| RT-PCR primer | TGFBR2-1-F/TGFBR2-1-R | F: TGCAGCGCCGAAGTGAAGTTTTC |
| R: TACATCTCCCTGCCCAGAGCCAC | ||
| TGFBR2-1-F/ TGFBR2-2-R | F: TGCAGCGCCGAAGTGAAGTTTTC | |
| R: GAACAGAGTCAGGCTGTGGTATG | ||
| TGFBR2-1-F/ TGFBR2-3-R | F: TGCAGCGCCGAAGTGAAGTTTTC | |
| R: GTCCGTAAAGACCACTCAACATA | ||
| TGFBR2-2-F/ TGFBR2-1-R | F: GGGGAGAAGGAACTACTGTAAGA | |
| R: TACATCTCCCTGCCCAGAGCCAC | ||
| TGFBR2-2-F/ TGFBR2-2-R | F: GGGGAGAAGGAACTACTGTAAGA | |
| R: GAACAGAGTCAGGCTGTGGTATG | ||
| TGFBR2-2-F/ TGFBR2-3-R | F: GGGGAGAAGGAACTACTGTAAGA | |
| R: GTCCGTAAAGACCACTCAACATA | ||
| PCR primer | Genome- F/R | F: AGAAGGATGATGGGGGACTGA |
| R: TGGTTTGAGCACGTTGTTGC | ||
| qRT-PCR primer | cTGFBR2-total-F/R | F: CTAAGAGACAGAGGGCGACC |
| R: ACAGCTTCTCCCTGAGAGCT | ||
| cTGFBR2-1-F/R | F: TAGAAGCAAGGAAAATGG | |
| R: CTGGCTTATGGGGATCAA | ||
| cTGFBR2-3-F/R | F: TTGGTCCTCTGGGAAATG | |
| R: AGATGGGCTTTGTAGTGC | ||
| TBP-F/R | F: GCGTTTTGCTGCTGTTATTATGAG | |
| R: TCCTTGCTGCCAGTCTGGAC |
Abbreviations: TGFBR2, transforming growth factor beta receptor Ⅱ; TBP, TATA-box binding protein.