Table II.
Details of the antibodies used in western blot analysis.
| Antibody | Dilution | Cat. no. | Manufacturer | Application |
|---|---|---|---|---|
| IGF1R | 1:1,000 | ab182408 | Abcam | Primary antibody |
| PI3K | 1:1,000 | ab191606 | Abcam | Primary antibody |
| AKT | 1:500 | ab8805 | Abcam | Primary antibody |
| p-PI3K | 1:500 | ab182651 | Abcam | Primary antibody |
| p-AKT | 1:500 | ab38449 | Abcam | Primary antibody |
| eNOS | 1:1,000 | PA1-037 | Invitrogen; Thermo Fisher Scientific, Inc. | Primary antibody |
| p-eNOS | 1:500 | MA5-14957 | Invitrogen; Thermo Fisher Scientific, Inc. | Primary antibody |
| Goat anti-rabbit | 1:20,000 | ab205718 | Abcam | Secondary antibody |
| GAPDH | 1:5,000 | ab181602 | Abcam | Loading control |
p, phosphorylated; eNOS, endothelial nitric oxide synthase; IGF1R, insulin-like growth factor 1 receptor.