FIG. 6.
LcrV is secreted via the type III pathway. (A) Y. enterocolitica wild-type strain W22703 (wt) and isogenic mutant strains CT1 (lcrV), MC2 (lcrG), VTL1 (yopN), KUM1 (yscV), NIA6 (yscD), and CT130 (yscN) were grown in presence of 5 mM calcium (+Ca2+) or 5 mM EGTA (−Ca2+) at 37°C. Cultures were centrifuged, and the supernatant (S) was separated from the cell pellet (P). Protein in each sample was precipitated with TCA, solubilized in sample buffer, and analyzed by immunoblotting with antisera raised against LcrV, YopE, and LcrH. (B) Immunoblots were quantified, and the amount of secreted polypeptide is recorded as a percentage.