FIG. 7.

Binding of Gst-LcrG to LcrV sequences. LcrV gene sequences were fused to the 3′-GST gene sequences or to the 5′ end of a gene encoding DHFR_His6. Recombinant proteins were purified, separated by SDS-PAGE, and electroblotted onto PVDF membrane in duplicate. One membrane was stained with Coomassie brilliant blue (A), whereas the other was incubated with ³²P-labeled GST-LcrG. Binding was quantified on a PhosphorImager instrument (B) and is reported in arbitrary units per picomoles of LcrV fusion protein (C). The migration of molecular mass markers (M) is indicated in kilodaltons.