TABLE 1.
Plasmids used in this work
| Plasmid | Encoded protein (promoter)a | Vector | Antibiotic resistanceb | Source or reference |
|---|---|---|---|---|
| pKD2 | LcrV-DHFRHis6 (T7)* | pET9a | Km | This work |
| pKD3 | LcrV1–200-DHFRHis6 (T7)* | pET9a | Km | This work |
| pKD4 | LcrV1–100-DHFRHis6 (T7)* | pET9a | Km | This work |
| pKD5 | LcrV1–50-DHFRHis6 (T7)* | pET9a | Km | This work |
| pKD6 | LcrV1–15-DHFRHis6 (T7)* | pET9a | Km | This work |
| pKD7 | LcrVΔ2–14-DHFRHis6 (T7)* | pET9a | Km | This work |
| pKD8 | LcrV-NPT (lcrG)* | pHSG576 | Cm | This work |
| pKD14 | LcrV-GST (sycE)* | pHSG576 | Cm | This work |
| pKD15 | FLAG-LcrV (tac)* | pHSG576 | Cm | This work |
| pKD16 | GST-LcrV201–326 (tac)* | pHSG576 | Cm | This work |
| pKD17 | GST-LcrV201–274 (tac)* | pHSG576 | Cm | This work |
| pKD18 | GST-LcrV275–326 (tac)* | pHSG576 | Cm | This work |
| pKD19 | GST-LcrV101–326 (tac)* | pHSG576 | Cm | This work |
| pKD20 | GST-LcrV101–274 (tac)* | pHSG576 | Cm | This work |
| pKD21 | GST-LcrV151–274 (tac)* | pHSG576 | Cm | This work |
| pKD22 | GST-LcrV51–326 (tac)* | pHSG576 | Cm | This work |
| pKD23 | GST-LcrV51–274 (tac)* | pHSG576 | Cm | This work |
| pKD24 | GST-LcrV151–326 (tac)* | pHSG576 | Cm | This work |
| pCT60 | LcrG (tac) | pHSG576 | Cm | This work |
| pVL48 | GST-LcrG (tac) | pHSG576 | Cm | This work |
| pGEX-2TK | GST (tac) | pHSG576 | Cm | 29 |
| pVL47 | GST-LcrV (tac) | pHSG576 | Cm | 33 |
| pVL69 | LcrV-Flag (tac) | pHSG576 | Cm | This work |
| pLC15 | YopQ-DHFRHis6 (T7) | pET9a | Km | 8 |
| pHSG576 | Low-copy cloning vector | Cm | 59 | |
| pET9a | T7 polymerase expression | Km | 58 |
a
For entries indicated with an asterisk, the primer sequences can be obtained from the authors upon request.
b
Km, resistance to 50 μg of kanamycin per ml; Cm, resistance to 20 μg of chloramphenicol per ml.