Fig. 3.

Application and validation of multiplex ROS-cell viability assay for HT studies in gut-on-a-chip model with Caco2 cultured as a tubule in the OrganoPlate 3-lane 40 against ECM. Gut-on-a-chip models were treated with standard media or 0.1% of DMSO or TBHP 10 mM with and without curcumin 67 μM, and stained for DNA with Hoechst (blue), for ROS with DHR123 (green) and dead cells with PI (red) (A) Representative sum-projection images of bottom part of tubule of Caco-2 with a zoom-in on the cells below. (B) ROS content in a log scale expressed as fluorescence intensity normalized against the number of cells count (C) Cell viability expressed as percentage. Data are expressed as average ± STD and derived from 3 separate experiments (N = 3) with n = 3–7. Difference between results were evaluated by one-way ANOVA with Tukey's multiple comparison. P-value < 0.05 was considered statistically significant ***p = 0.0003, ****p < 0.0001. All scale bars are 100 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)