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Non-targeted IM-MS analysis of released glycans from hAGP. (A) MS of native glycans shows nine complex-type, sialylated species. Without prior quadrupole isolation, CID activation leads to the fragmentation of all ionized glycans. The resulting B3 fragment results from all sialylated glycans. (B) The ATD of the B3 fragment represents the averaged drift time of α2,6- and α2,3-linked sialic acids for all sialylated glycans and can be used to estimate the overall sialic acid ratio.
