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. 2022 Sep 22;13:1005332. doi: 10.3389/fimmu.2022.1005332

Figure 1.

Figure 1

Optimization of the Expression of Tag-free CyRPA. (A) Different E. coli strains- BLR (DE3), BL21 (DE3) and Shuffle 26 (DE3) were tested for the expression of tag-free CyRPA. CyRPA expression was analyzed in the induced samples both in the supernatant and pellet fraction by Coomassie stained reducing SDS-PAGE. CyRPA was observed in all the three strains with BL21 (DE3) inducing the highest expression. (B) The expression of tag-free CyRPA as shown in (A) was confirmed by immunoblotting using anti-CyRPA rabbit polyclonal sera. Consistent with SDS-PAGE results, anti-CyRPA sera confirmed tag-free CyRPA expression in all the three E. coli strains. * indicating the position of CyRPA. I, Induced; S, Supernatant; P, Pellet; S26, Shuffle 26.