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. Author manuscript; available in PMC: 2022 Dec 1.
Published in final edited form as: Exp Neurol. 2021 Aug 26;346:113845. doi: 10.1016/j.expneurol.2021.113845

Figure 6. Encephalitic infection with WEEV induces opsonization of α-synuclein protein aggregates in the cortex, hippocampus and midbrain with astrocyte-derived complement C3 protein.

Figure 6.

Immunofluorescence images of complement C3b (red) co-localization with P129+ protein aggregates (green) were analyzed in the cortex (A-C), hippocampus (D-F) and midbrain (G-I) at various times following infection with WEEV. Nuclei were counterstained with DAPI (blue). High magnification 40X inset images are depicted from each region. Representative images panels are presented from 2 WPI, the time of maximal P129 aggregation. (J) Quantification of C3b deposition co-localizing with protein aggregates within each brain region was determined at 2, 4 and 8WPI by analyzing the amount of C3b co-localizing with regions of interest detecting P129+ aggregates. (*p<0.05, **p<0.005, ***p<0.0005, n=6-8 per group)