FIG. 4.

Northern blot analysis of mRNA expression of the five cloned histone deacetylase genes of C. albicans strain WO-1 in the white (Wh) and opaque (Op) phases. Blots were probed with the full-length ORF of each of the five deacetylase genes. The ethidium bromide-stained 18S rRNA band is presented at the bottom of the hybridization patterns to assess loading. Molecular sizes of the bands are presented to the right of each blot. Note that HOS3 is expressed as an abundant 2.5-kb message in the white phase and a far-less-abundant 2.3-kb transcript in the opaque phase.